Contact : Anne Chantal Gouget-Laemmel
Participants : Anne Chantal Gouget-Laemmel, F. Ozanam
The fast and reliable identification of pathogens at trace levels is a major issue in many areas including health or food safety. Biosensors are promising tools for the fast and cost-effective detection of a given pathogen thanks to the specific interactions between biological probes grafted on the biosensor surface and pathogenic targets from a sample to be analyzed. However, these tools lack reliability, and in the case of bacteria, they do not allow the identification of the nature of bacterial strains.
Since 2010, we have been working on implementing amorphous silicon-based optical biochips to improve reliability and sensitivity and the possibility of multiplex detection. Our approach is to develop a robust and reproducible surface bioengineering on hydrogenated crystalline silicon model surfaces (based on Si-C bonds formation) to observe selective biomolecular interactions without uncontrolled physisorption. Quantitative surface analyses are performed using ATR-IR spectroscopy but also XPS, colorimetry and AFM imaging to optimize the density and orientation of probes and their interactions with targets. Then, these well-controlled chemical processes are transferred to the biosensor’s architectures based on amorphous silicon (hydrogenated a-Si:H or carbon alloy a-Si1-xCx:H) deposited on glass, leading to increased reliability. Optical detection methods are chosen such as fluorescence, coupled to (localized) surface plasmons and surface exalted Raman scattering (SERS) because of their very high sensitivity to detect traces of targets.
Representation of an antibody biochip based on a-Si:H deposited on glass and on gold nanorods for the selective identification of the uropathogenic bacteria Escherichia coli by SERS imaging. A very low concentration of 10 CFU/mL (well below the inflammatory threshold in humans) was identified in artificial urine in less than 3 hours (vs 2 days in biomedical laboratory)
Previous PhD thesis : Cassiana Andrei (DGA, 2019)
Collaborations : ITODYS (Paris), IEMN (Lille)
Fundings : DGA
Recent publications :
[Andrei2020] Andrei, C. C., Moraillon, A., Lau, S., Felidj, N., Yamakawa, N., Bouckaert, J., Larquet, E., Boukherroub, R., Ozanam, F., Szunerits, S., Gouget-Laemmel, A. C., Rapid and sensitive identification of uropathogenic Escherichia coli using a surface-enhanced-Raman-scattering-based biochip, TALANTA, 2020, 219, 121174
https://doi.org/10.1016/j.talanta.2020.121174
[Andrei2021] Andrei, C. C., Moraillon, A., Larquet, E., Potara, M., Astilean, S., Jakab, E., Bouckaert, J., Rosselle, L., Skandrani, N., Boukherroub, R., Ozanam, F., Szunerits, S., Gouget-Laemmel, A. C., SERS characterization of aggregated and isolated bacteria deposited on silver-based substrates, ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2021, 413, 1417-1428
https://doi.org/10.1007/s00216-020-03106-5
[Aschl 2020] Aschl, T., Frison, G., Moraillon, A., Ozanam, F., Allongue, P., Gouget-Laemmel, A. C., Insights into the Ochratoxin A/Aptamer Interactions on a Functionalized Silicon Surface by Fourier Transform Infrared and UV-Vis Studies, LANGMUIR, 2020, 36, 13908-13917
https://doi.org/10.1021/acs.langmuir.0c02358